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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Thorac Oncol. 2014 Apr;9(4):519–526. doi: 10.1097/JTO.0000000000000111

Figure 1. Differential effects of Dex on the expression of genes involved in pemetrexed action in A549 vs. H1299 cells.

Figure 1

A549 cells (Panels A, C) and H1299 cells (Panels B, D) were plated at 20 percent confluence in media containing charcoal-stripped serum for 24 h for hormone depletion. Cells were then treated with either vehicle (ethanol) or Dex (100 nM) for 48 h. Cells were harvested and total RNA extracted to measure mRNA levels by real time RT-PCR (Panels A and B). Whole cell lysates were also extracted from the cells to measure the protein levels of TS and DHFR by western blot; GAPDH was used as the loading control (Panels C and D). * P < 0.01. All of the mRNA measurements were carried out using biological triplicate samples.