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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Thorac Oncol. 2014 Apr;9(4):519–526. doi: 10.1097/JTO.0000000000000111

Figure 4. Effect of restoring GRα on responsiveness to Dex in H1299 cells.

Figure 4

H1299-GRα cells were generated as described under Materials and Methods. RNA extracted from A549 cells, H1299 cells and H1299-GRα cells was used to measure the relative mRNA levels for GRα (Panel A). H1299-GRα cells were plated at 20 percent confluence in media containing charcoal-stripped serum for 24 h for hormone depletion. Cells were then treated with either vehicle (ethanol) or Dex (100 nM) for 48 h. Cells were harvested for mRNA measurement by real time RT-PCR (Panel B). * P < 0.01. In parallel, cells were harvested for cell cycle analysis (Panel C). The experiments were repeated at least three times. Within each experiment, the percent error for replicate samples was < 10 percent. The P values for Dex induced changes noted in the text were < 0.0001. All of the mRNA measurements were carried out using biological triplicate samples.