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Enhanced stimulation of TIL after cross-presentation of NP encapsulated tumor antigens by autologous DC. Antigen loaded-DC and TIL were co-incubated overnight and supernatants collected and analyzed for Tcell production of IFN-γ and IL-10. NP-mediated antigen delivery led to: (a) increased immunostimulatory IFN-γ (*p=0.002 versus lysate and #p=0.035 versus blank-NP) and (b) decreased immunoinhibitory IL-10 (*p=0.002 versus lysate and #p=0.015 versus blank-NP).
