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. 2014 Jun 5;16(3):R120. doi: 10.1186/ar4575

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Copyright © 2014 Bengtsson et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Reactive oxygen species (ROS) production in polymorphonuclear leukocytes (PMN) from systemic lupus erythematosus (SLE) patients did not correlate with Hydroxychloroquine treatment or the dose of prednisone. ROS production was measured by flow cytometry after ex vivo activation of PMN with phorbol 12-myristate 13-acetate (A) the amount ROS produced by SLE-PMN, treated with or without Hydroxychloroquine. (B) The amount of ROS produced in individual patients plotted against the dose of prednisone at the day of sampling. The amount ROS produced is shown as geometric mean fluorescence intensity (geo mean fluorescence, MFI). The line represents the median value of each dataset.