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GREtkLUC acts as an accelerator at the CLS in assay with nocodazole (Nocod) acting as an accelerator after the CLS. Values of Amax and EC50 from competition assays of the indicated amounts of GREtkLUC and nocodazole were determined and plotted as 1/EC50 vs GREtkLUC (A), Amax/EC50 vs GREtkLUC (B), and Amax/EC50 vs nocodazole (C) as described in Materials and Methods. Similar results were obtained in 2 additional independent experiments.
