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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Virology. 2014 May 14;0:172–182. doi: 10.1016/j.virol.2014.04.036

Figure 4.

Figure 4

Purified sNS1 inhibits TLR signaling in BMDMs and BMDCs. (A) BMDMs derived from C57BL/6 mice were incubated for 16h with 5μg/mL sNS1 or sNS1-heat, or pCtrl followed by 8h pIC treatment at 5μg/mL. IL-6 release was measured by ELISA. (B) Myeloid DCs derived from WT C57BL/6 mice were incubated with 5μg/mL sNS1 or controls for 16h and stimulated with 10μg/mL pIC for 12h. IL-12p70 release into supernatants was determined by ELISA. Control for these experiments was supernatant from 293T vector control cells that underwent an identical purification process as NS1 supernatants and was added at the same volume as sNS1. Asterisks denote statistically significant differences between sNS1-heat and sNS1 pretreated -stimulated cells.