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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Virology. 2014 May 14;0:172–182. doi: 10.1016/j.virol.2014.04.036

Figure 6.

Figure 6

Secreted NS1 associates with innate cells and inhibits PRR signaling in vivo. (A) 5μg of Alexa 488 fluorescently labeled purified sNS1 protein were injected into mice via the rear footpad, After 6h draining lymph nodes were harvested and analyzed for cell type specific markers and NS1 fluorescence. The percentage of NS1 positive cells in the entire cell type population is shown. (B and C) C57BL/6 mice were injected with 5μg of purified sNS1 into each rear footpad. After 6 hours the mice were stimulated by injection of 20μg of Poly(IC:LC) per footpad. After 16h pLNs were harvested and pooled per mouse before RNA extraction and cDNA synthesis. IFNβ mRNA levels (B) and IL-6 mRNA levels (C) were determined by real-time qRT-PCR. Controls for the experiments in (B) and (C) were heat-inactivated sNS1 and the purification control.