Extended Data Figure 7. BBS4 recruitment to primary cilia is defective in Atg5^−/− MEFs.

a, Representative confocal images of Atg5^+/+ and Atg5^−/− MEFs expressing Myc-BBS4 subjected to 24-hour serum starvation. Scale bar 5 μm. b, Quantified percentage of cells with Myc-BBS4 translocation into primary cilia in Atg5^+/+ and Atg5^−/− MEFs. Data shown represent mean ± s.d. for 100 cells per well in triplicate samples. ***P<0.001, two-tailed unpaired student’s t-test. a, b, Similar results were obtained in three independent experiments.