Extended Data Figure 10. Partial knockdown OFD1 in MCF7 cells depletes OFD1 from centriolar satellites and promotes primary ciliogenesis.

a, b, OFD1 was depleted from centriolar satellites in OFD1 shRNA knockdown MCF7 cells. a, Representative confocal images of relative localization of OFD1 with axoneme marker acetylated tubulin in MCF7 OFD1 Knockdown clone (C19). Data shown represent for 100 cells per well in triplicate samples. Scale bar 5 mm. b, Representative confocal images of OFD1 and centriole marker g-tubulin from C19. Data shown represent for 100 cells per well in triplicated samples. Scale bar 10 mm. c, Quantified percentage of parental MCF7 and C19 cells with centriolar satellite OFD1. Data shown represent mean ± s.d. for 100 cells per well in triplicate samples. ***P<0.001, two-tailed unpaired student’s t-test. d, e, f, Primary cilia formed in OFD1 knockdown C19 MCF7 cells are positive for cilia markers. Representative confocal images of primary cilia formed in C19 subjected to 72-hour serum starvation. Cilia were positive for ciliary membrane marker Arl13b, axoneme marker acetylated tubulin and intraflagellar transport protein IFT88. a–f, Similar results were obtained in three independent experiments.