Fig. 2. Autophagy specifically degrades OFD1 at centriolar satellites upon serum starvation.
a, Representative confocal images of OFD1 puncta with cilium marker acetylated tubulin, or centriole marker γ-tubulin in hTERT-RPE1 cells in normal medium or subjected to 24-hour serum starvation. b, Representative confocal images of endogenous Ofd1 and acetylated tubulin in Atg5+/+ and Atg5−/− MEFs subjected to 24-hour serum starvation. a, b, Data shown represent mean ± s.d. percentage of cells with centriolar satellite OFD1 for 100 cells per well in triplicate samples. ***P<0.001, two-tailed unpaired student’s t-test. Similar results were observed in three independent experiments.