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. Author manuscript; available in PMC: 2014 Jun 30.
Published in final edited form as: Nature. 2013 Oct 2;502(7470):254–257. doi: 10.1038/nature12606

Fig. 4. Forced OFD1 reduction promotes ciliogenesis in human breast cancer MCF7 cells.

Fig. 4

a, Western blotting analysis of OFD1 protein levels in control or OFD1 knockdown MCF7 (C-19) cells, quantified Ofd1 level was normalized with β-tubulin. b, Quantification of percentage of cells with primary cilia in MCF7 or C19 cells subjected to 72-hour serum starvation. Data shown represent mean ± s.d. percentage of cells with primary cilia for 500 cells per well in triplicate samples. c, Quantification of length of primary cilia in C19 cells subject to 48-hour or 72-hour serum starvation. d, e, Representative confocal images of primary cilia with variable length formed in MCF7 or C19 cells subject to 72-hour serum starvation. Data shown represent for 100 cells per well in triplicate samples. f, Scanning electron microscope analysis of primary cilia (marked by arrows) formed in C-19 cells subject to 72-hour serum starvation and cycling Atg5+/+ MEFs in normal medium for 24-hour. In a–e, similar results were observed in three independent experiments.

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