Extended Data Figure 2. PCM1 is required for OFD1 centriolar satellite localization.

a, Representative confocal images of OFD1 and PCM1 localization from control or PCM1 knockdown U₂OS cells in normal medium. Data shown represent for 100 cells per well in triplicate samples. Scale bar, 10 mm. b, Quantified percentage of cells with PCM1 positive centriolar satellite OFD1 in a. Data shown represent 100 cells per well in triplicated samples. ***P<0.001, two-tailed unpaired student’s t-test. c, LC3 partially colocalizes with PCM1 upon serum starvation. Representative confocal images of Myc-LC3 and PCM1 colocalization in U₂OS cells expressing Myc-LC3 in normal medium or subjected to 24-hour serum starvation. Arrows denote colocalized LC3 (green) and PCM1 (red) puncta. Data shown represent for 100 cells per well in triplicate samples. Scale bar, 5 mm. d, Quantified percentage of cells with colocalization of Myc-LC3 and PCM1 in c. ***P<0.001, two-tailed unpaired student’s t-test.