Skip to main content
. Author manuscript; available in PMC: 2014 Jun 30.
Published in final edited form as: Nature. 2013 Oct 2;502(7470):254–257. doi: 10.1038/nature12606

Extended Data Figure 5. The turnover rate of centriolar satellite Ofd1 is faster than Ofd1 at centrioles.

Extended Data Figure 5

a, Centriolar satellite Ofd1 has a shorter half-life compared to Ofd1 at centrioles. Quantified percentage of cells with Ofd1 at centrioles or at centriolar satellites from Atg5+/+ and Atg5−/− MEFs in normal medium or subjected to 75 μM cycloheximide (CHX) with indicated time points. Data shown represent for 200 cells per well in triplicate samples. b, Centriolar satellite Ofd1 but not centriole Ofd1 degrades upon serum starvation. Quantified percentage of cells with Ofd1 at centrioles or at centriolar satellites from Atg5+/+ and Atg5−/− MEFs in normal medium or subjected to serum starvation with indicated time points. Data shown represent for 200 cells per well in triplicate samples. a, b, Similar results were obtained in three independent experiments.

HHS Vulnerability Disclosure