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. Author manuscript; available in PMC: 2014 Jun 30.
Published in final edited form as: Dev Biol. 1992 Oct;153(2):206–216. doi: 10.1016/0012-1606(92)90106-q

TABLE 1.

Distribution of Myogenic Cells Reacting with Anti-ventricular and/or Anti-embryonic MHC mAbs

Source of cells Culture age (days) Total nuclei Number of fields Nuclei in E+V+ cells
Nuclei in V+ cells
Total+ cells
Myotube Myocyte Myotube Myocyte Myotube Myocyte
E14 1 500 47 4 30 2 4 6 34
510 45 4 24 0 0 4 24
2 516 12 12 34 0 13 12 47
530 15 9 24 4 6 13 30
3 967 10 499 39 0 18 499 57
752 10 426 39 3 6 429 45
4 981 10 466 6 10 4 476 10
1359 10 628 3 3 7 631 10
5 1836 10 824 8 19 22 843 30
2206 10 1061 6 24 20 1085 26
E18 1 501 42 0 0 0 0 0 0
504 42 0 0 0 0 0 0
2 506 31 0 0 0 2 0 2
511 28 0 0 0 0 0 0
3 505 14 3 0 0 3 3 3
501 15 0 0 0 0 0 0
4 1097 10 0 0 11 28 11 28
942 10 0 2 4 29 4 31
4.5 1518 10 30 1 184 75 214 76
1445 10 37 0 160 45 197 45
5 1635 10 475 12 382 59 857 71
2009 10 694 5 349 53 1043 58
6* 552 10 183 0 3 4 186 4
572 10 207 1 0 1 207 2
1-day 1 500 104 0 0 0 0 0 0
501 95 0 0 0 0 0 0
2 504 60 0 0 0 0 0 0
506 69 0 0 0 0 0 0
3 503 25 0 0 0 0 0 0
519 26 0 0 0 0 0 0
4 507 12 0 0 0 0 0 0
525 15 0 0 4 3 4 3
5 1002 10 0 0 0 35 0 35
814 10 0 0 0 22 0 22
6 3202 10 17 1 314 87 331 88
2097 10 0 0 151 87 151 87
7 3637 10 219 0 1249 59 1468 59
3632 10 381 1 1073 35 1454 36
8* 688 10 195 1 37 3 232 4
897 10 234 1 11 0 245 1
3-week 1 524 37 0 0 0 8 0 8
500 39 0 0 0 0 0 0
2 505 22 0 0 2 9 2 9
513 19 0 0 0 1 0 1
3 926 10 0 0 0 12 0 12
875 10 0 0 2 11 2 11
4 2296 10 0 0 383 81 383 81
2258 10 0 0 330 79 330 79
5 3072 10 870 0 954 22 1824 22
2726 10 437 0 1244 20 1681 20
6* 893 10 195 0 4 10 109 10
867 10 260 0 3 7 260 7

Note. Duplicate cultures were fixed and double-labeled at each time point with mAbs against embryonic fast MHC (EB165 indirectly labeled with GAM-TRITC) and ventricular MHC (HV11 directly labeled with fluorescein). Nuclei were counterstained with DAPI and the number of nuclei within MHC-positive cells versus total nuclei was determined using a 40X objective, except where indicated (*), when counts were determined using a 100X objective.