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. 2014 Jul 1;127(13):2920–2933. doi: 10.1242/jcs.148130

Fig. 1.

Fig. 1.

p27−/− MEFs exhibited elevated directional and random cell migration capability. (A) Assessment of p27 protein expression in p27+/+ and p27−/− MEFs. (B–E) The wound-healing assay (B,C) and transwell assay (D,E) were used to determine the directional cell migration of p27+/+ and p27−/− MEFs. (F,G) p27+/+ and p27−/− MEFs were pretreated with mitomycin C (10 µg/ml) for 3 h, and the cells were then used in the wound-healing assay (F) and transwell assay (G). (H,I) Cell migration was monitored using time-lapse microscopy. The migration speed, expressed as average speed of cell locomotion (ASL, left panel) and average rate of cell displacement (ARD, right panel), was compared between p27+/+ and p27−/− MEFs using ImageJ software. *P<0.05 (between p27+/+ and p27−/− MEFs). The data shown are representative of three independent experiments.