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. 2014 Jul 1;127(13):2920–2933. doi: 10.1242/jcs.148130

Fig. 3.

Fig. 3.

Overexpression of GFP–p27 in HT1197 cells repressed cell migration. (A,B) p27 protein expression level was determined in different human bladder cancer cell lines by western blotting. (C) Plasmid encoding GFP-tagged human p27 was stably transfected into HT1197 cells, and the transfection efficiency was validated by western blotting. (D–F) The wound-healing assay (F) and transwell assay (D,E) were used to determine the cell migration of GFP–p27 and vector-control transfectants. Data show the mean±s.d. (three independent experiments); *P<0.05 (between GFP–p27 and vector-control transfectants).