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p27 inhibited MnSOD expression and maintained redox homeostasis in MEFs. (A,B) O2•− and H2O2 levels were evaluated by using hydroethidium and DCFH-DA staining in p27+/+ and p27−/− MEFs. Data show the mean±s.d.; *P<0.05 (between p27+/+ and p27−/− MEFs). (C–F) Protein expression levels of MnSOD and catalase were determined in the indicated cell lines by western blotting.
