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. 2014 Jul 1;127(13):2920–2933. doi: 10.1242/jcs.148130

Fig. 5.

Fig. 5.

MnSOD was responsible for the elevated migration of p27−/− MEFs. (A) MnSOD-specific shRNA was stably transfected into p27−/− MEFs, and the knockdown efficiency was determined by western blotting. (B) The levels of O2•− (upper panel) and H2O2 (lower panel) were evaluated in shRNA-MnSOD and non-silencing control transfectants. (C) The regulatory effect of MnSOD-specific shRNA on cell migration was examined by using the wound-healing assay. *P<0.05 (between shRNA-MnSOD and non-silencing control transfectants). (D) Catalase and Mito-Catalase were stably transfected into p27−/− MEFs, and their expression was validated by western blotting. (E) The H2O2 level was evaluated in catalase and Mito-Catalase transfectants in the absence and presence of 100 µM H2O2. *P<0.05 (between H2O2-treated and medium control); P<0.05 (between catalase and vector-control transfectants in the presence of H2O2). (F,G) The effect of catalase and Mito-Catalase overexpression on cell migration was determined by using the wound-healing assay. *P<0.05 (between catalase overexpression and vector-control transfectants). Data show the mean±s.d.