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. 2014 Jul 1;127(13):2920–2933. doi: 10.1242/jcs.148130

Fig. 7.

Fig. 7.

p27 suppressed STAT3 transcription by targeting CRE-dependent but not SBE-dependent transcriptional activity. (A) The level of Stat3 mRNA was compared between p27+/+ and p27−/− MEFs by RT-PCR. (B) Stat3 mRNA stability was compared between p27+/+ and p27−/− MEFs in the presence of 5 µM Act D for the indicated time periods. (C,D and G–I) The luciferase activities of full-length and various mutated versions of the Stat3 promoter (C,D) and CRE-transactivation (G–I) was evaluated in p27+/+ and p27−/− MEFs. *P<0.05 (between p27+/+ and p27−/− MEFs). (E,F) Stat3 mRNA level and full-length Stat3-promoter-driven luciferase activity were assessed in shRNA-p27 and non-silencing transfectants. *P<0.05 (between shRNA-p27 and control non-silencing transfectants). Data show the mean±s.d.