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Coimmunoprecipitation of core gypsy insulator proteins with Rump. Nuclear extracts from mixed stage embryos (lane 1) were immunoprecipitated (IP) with either serum against Rump (lanes 2 and 4) or pre-immune serum (lanes 3 and 5). Unbound (lanes 2–3) and bound fractions (lanes 4–5) are shown. All of the indicated proteins were detected by western blotting. Approximately 2.4% of the total Rump and 0.2% of each total insulator protein was immunoprecipitated.
