C57BL/6J mice were treated i.t. with a mixture of LTA (2.5 mg/kg) and PepG (2.5 mg/kg), with or without concurrent i.v. treatment with Y27632 (2 mg/kg), SB203580 (10 mg/kg) for 24 hours. Control animals were treated with sterile saline solution. A - Protein concentration; B - Total cell count; and C - Neutrophil count were measured in bronchoalveolar lavage fluid taken from control and experimental animals, n=6 per condition; *p<0.05. D – Histological analysis of lung tissue (×40 magnification). Whole lungs (4 to 6 animals from each experimental group) were agarose-inflated in situ, fixed with 10% formalin, and used for hematoxylin and eosin staining and histological evaluation.