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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Transl Res. 2013 Apr 6;162(1):45–55. doi: 10.1016/j.trsl.2013.03.005

Figure 2. Effects of combined LTA and PepG treatment on inflammatory cascade activation in pulmonary macro- and micro-vascular endothelium.

Figure 2

Figure 2

HPAEC (A) or HLMVEC (B) were treated with a combination of LTA (50 ng/ml) and PepG (100 ng/ml) for indicated periods of time. Phosphorylation of p38 MAPK, Hsp27, MYPT1, and MLC was determined by western blot with corresponding phospho-specific antibodies. Degradation of IκBα was detected using pan IκBα antibodies. Phosphorylation of the protein of interest was evaluated by quantitative densitometry and normalized to the total content of corresponding protein in cell lysates. Rate of IκBα protein degradation was normalized to β-tubulin content in cell lysates. Bar graphs represent results of three to six independent experiments; *p<0.01 vs vehicle control.