Table 1. Affinities of Prp8 fragments to full-length Brr2 proteins.
hPrp8 fragment | Kd (nM) | yPrp8 fragment | Kd (nM) | Type of RP13-linked mutation |
---|---|---|---|---|
hPrp8Jab1 | 7.7 | yPrp8Jab1 | 7.6 | - |
hPrp8Jab1-Q2321stop | 3.2 | - | - | III |
- | - | yPrp8Jab1-ΔC16 | 11.1 | - |
hPrp82300–2335 | 3150.0 | yPrp82378–2413 | 930.0 | - |
hPrp8Jab1-R2310G | 81.3 | - | - | II |
hPrp8Jab1-R2310K | 46.0 | - | - | II |
hPrp8Jab1-F2314L | 63.5 | - | - | II |
hPrp8Jab1-Y2334N | 20.0 | - | - | III |
For experimental details, see legend to Figure 4.