. 2014 Mar 5;11(4):298–312. doi: 10.4161/rna.28353

Table 2. Effects of yPrp8^Jab1 variants on the efficiency of yBrr2.

Proteins	ATP hydrolysis [nM min⁻¹]	U4/U6 unwinding [nM min⁻¹]	ATP hydrolyzed per U4/U6 unwound	Type of RP13-like mutation
yBrr2	15860 ± 2315	1.18	13441 ± 1962	-
+ yPrp8^Jab1	864 ± 103	2.80	313 ± 37	-
+ yPrp8^Jab1-ΔC16	27520 ± 358	18.0	1528 ± 20	-
+ yPrp8^Jab1-R2388K	8926 ± 1083	0.50	17853 ± 2165	II
+ yPrp8^Jab1-F2392L	3202 ± 301	0.95	3370 ± 317	II
+ yPrp8^{Jab1-A2399stop}	27317 ± 455	16.30	1676 ± 28	III
+ yPrp8^Jab1-F2412N	6868 ± 1994	12.95	530 ± 153	III

ATP hydrolysis and U4/U6 unwinding were monitored as described.⁴² For comparison of apparent unwinding efficiencies, ATP hydrolysis and U4/U6 unwinding were measured under identical conditions (200 nM yBrr2, 50 nM U4/U6 duplex, without or with 0.75 µM of the indicated yPrp8^Jab1 variants, 30 °C).

Table 2. Effects of yPrp8Jab1 variants on the efficiency of yBrr2.

Table 2. Effects of yPrp8^Jab1 variants on the efficiency of yBrr2.