Table 1.
Biomass and maximal scopularide A production by S. brevicaulis LF580 in bioreactor cultivations
| Condition | Glucose (g L -1 ) | C/N (g g -1 ) | Biomass (g L -1 ) | Scopularide A (mg L -1 ) † | Time (h) |
|---|---|---|---|---|---|
| Static flask - YMP [8] |
10 |
10 |
|
7 |
~336 |
| O2-limited batch-YMP†† |
11 |
10 |
11 |
10 |
90 |
| Batch – YMP |
11 |
10 |
11 ± 1 |
29 ± 3 |
90 |
| N-limited batch-YMP |
21 |
15 |
12 ± 1 |
8 |
74 |
| Batch – YMP |
20 |
11 |
15 ± 2 |
58 ± 4 |
90 |
| Batch or fed-batch-YMP |
44 |
10 |
25 ± 1 |
142 ± 20 |
167 |
| Batch or fed-batch-YMP |
61 |
10 |
33 ± 1 |
202 ± 58 |
165 |
| Batch – CSS |
17 |
7 |
12 |
44 |
90 |
| Batch – defined, glucose |
17 |
9 |
12 |
41 |
87 |
| Batch – defined, glucose |
40 |
9 |
24 ± 6 |
44 ± 7 |
100 |
| Batch – defined, xylose | 40 | 9 | 25 ± 1 | 54 ± 2 | 100 |
S. brevicaulis LF580 was grown in YMP, CSS or defined medium (and 30 g Sea Salt L-1) in batch or fed-batch culture at 28°C. Scopularide A was extracted from the mycelia at the times indicated. C/N values are estimated, as indicated in the methods. Average values are given, with ± sem for scopularide A and biomass, for cultivations which were carried out in replicate (n = 2 to 5). Published data from static flasks [8] is provided for comparison.
†Determined from total dry biomass, volume of culture remaining and specific scopularide A content of the biomass (see Figure 1).
††O2-limited culture agitated at 300 rpm, with 0.6 vvm air.