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. 2013 May 20;35(1):82–88. doi: 10.1038/aps.2013.31

Figure 2.

Figure 2

Evaluation of PAK5 gene silencing by transfection of cells with lentiviral shRNAs. Two lentiviruses, Lv-PAK5-shRNA1 and Lv-PAK5-shRNA2, each expressing a different shRNA against PAK5, were generated. A control lentivirus expressing a scrambled shRNA, Lv-scr-shRNA, was also prepared. HepG2 cells were transfected with the lentiviruses and PAK5 gene expression was evaluated after 72 h. (A) GFP expression was examined in transfected HepG2 cells by immunofluorescence (right column) (n=5. Scale bar=20 μm). The phase contrast images are also shown (left column). (B and C) PAK5 expression was evaluated in transfected HepG2 cells by qPCR (B) and Western blotting (C) (n=5). In panel (B), qPCR data are plotted as the mRNA fold change compared to Lv-scr-transfected cells, which was set at 1. bP<0.05.