Figure 2.

Carbachol-induced contractions are reduced by HA1100 and ML-9. (A) The upper trace (starting at 9 h following cystectomy) shows contractions induced by carbachol (CCh, 2 μmol/L) in a human detrusor specimen that were reduced by the ROCK blocker HA1100 (10 μmol/L) or combined pre-treatment with HA1100 and the MLCK blocker ML-9 (10 μmol/L). Note the reduction of the basal tone (arrow heads) indicating kinase activity at rest. The middle trace shows a similar experiment with another specimen from the same patient demonstrating that both ML-9 alone and combined application of ML-9 and HA1100 reduced the carbachol-induced contraction. The lower trace shows time-control experiments of another specimen from the same patient demonstrating only little tachyphylaxis upon repetitive carbachol applications. Application of KCl (60 mmol/L) was used at the beginning and at the end of the experiment to verify stable recording conditions. (B) Carbachol-induced contractions (in % of initial response to KCl 60 mmol/L) showed little tachyphylaxis (time-control experiments), but were significantly reduced following HA1100 or ML-9 treatment (total number of specimens: n=40 of 4 patients). ^bP<0.05, ^cP<0.01.