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. 2013 Oct 14;35(1):58–64. doi: 10.1038/aps.2013.122

Figure 3.

Figure 3

MGO affected the single-channel properties of the KATP channel. (A) In a giant inside-out patch configuration, 3 mmol/L MGO activated the Kir6.1/SUR2B currents in a manner similar to that seen in the whole cell configuration. This current could be further activated and inhibited by Pin and Glib, respectively. (B) Summary of the MGO treatment in the inside-out patch study. bP<0.05. (C) Single-channel level currents were recorded in inside-out patches with a holding potential of -80 mV. The lower trace was an expansion from the upper trace between the arrows. The presence of two active channels was evident at the baseline level. The NPO averaged 0.183 (n=4) in the baseline. (D) Treatment of the inside-out patch with 3 mmol/L MGO augmented the single-channel activity, as a result of the appearance of one more active channel. The NPO averaged 0.444 with MGO treatment. (E, F) Single-channel conductance was measured with a ramp protocol with voltage ranging from -100 mV to 100 mV. Three active channels were observed with both MGO alone (E) and Pin alone (F) treatments. The straight lines represent a slope conductance of 36 pS for all three channels.