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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2014 Apr 13;72:296–307. doi: 10.1016/j.freeradbiomed.2014.04.004

Fig. 5. ROS and p53 are involved in chrysotile asbestos-induced autophagy.

Fig. 5

(A) Western blot of p-JNK in control cells, cells treated with 100 μg/cm2 chrysotile asbestos or 100 μg/cm2 chrysotile asbestos plus 5 mM NAC for 24 h. (B) Western blot of LC3 in control cells, cells treated with 100 μg/cm2 chrysotile asbestos or 100μg/cm2 chrysotile plus 30 μM pifithrin-α for 5 h. The ratio of LC3-II/β-tublin was determined. The intensity of these protein signals obtained was quantified using Image J software from three replicate experiments.