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. 2014 Jun 9;9:16. doi: 10.1186/1749-8546-9-16

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Copyright © 2014 Tang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Intracellular DOX accumulation in breast cancer cells. Cells were pretreated with 5 μM DOX, 10 μM PD, or 5 μM DOX + 10 μM PD for 1 h. Then, the cells were washed, harvested, and re-suspended in PBS. Intracellular fluorescence was measured by flow cytometry analysis. The fluorescence intensity indicates the intracellular DOX concentration. (A) Flow cytometry assay images of MCF-7 cells; (B) statistical results of three independent tests of A; (C) flow cytometry assay images of MDA-MB-231 cells; and (D) statistical results of three independent tests of C. Values were expressed as mean ± SD of three independent assays. Statistical analysis was performed with one-way ANOVA and Tukey’s test.