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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Nat Struct Mol Biol. 2014 May 4;21(6):528–534. doi: 10.1038/nsmb.2820

Figure 3. Disruption of complex formation affects spacer acquisition in vivo.

Figure 3

(a) A close-up view of the Cas1a–Cas2 protein-protein interface, with annotations for the residues involved in electrostatic interactions. (b) View of the ordered C-terminal tail of Cas1a with the electron density mesh contoured at 1.0 sigma. (c–e) Agarose gels of in vivo acquisition assays with mutations of Cas1 and Cas2 at the C-termini (c) and the electrostatic interface (d,e). (f) Western blot of FLAG immunoprecipitations in BL21-AI cells expressing Cas1-FLAG and Cas2-HA or various mutations of Cas1 and Cas2. Despite the low expression of Cas2 E65R, we still detect its co-elution with Cas1.