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. Author manuscript; available in PMC: 2014 Dec 12.
Published in final edited form as: Nature. 2014 Apr 23;510(7504):278–282. doi: 10.1038/nature13229

Figure 1. Prostate cancer cell lines with intact androgen signaling are sensitive to BET bromodomain inhibition.

Figure 1

a, IC50 for JQ1 in each cell line is listed. b, Induction of apoptosis in VCaP prostate cancer cells by JQ1. Cleaved PARP (cPARP) immunoblot analysis. GAPDH served as a loading control. c, QRT-PCR analysis of indicated genes in VCaP treated with varying concentrations of JQ1 for 24hrs. Data represent mean ±S.E. (n=3) from one of the three independent experiments. d, Immunoblot analyses of AR, PSA and ERG levels in VCaP treated with JQ1. e, GSEA of the AR target gene signature in VCaP, LNCaP, 22RV1, and DU145 cells. NS, not-significant, *P ≤ 0.05, **P ≤ 0.005 by two-tailed Student's t-test.