Figure 7.

Effect of CMKLR1 point mutations on the agonist-induced phosphorylation of ERK 1/2. Transiently transfected HeLa cells expressing either WT or mutant CMKLR1 receptors were harvested 36 h after transfection. The cells were stimulated with 10 nmol/L C9 at 37 °C for the indicated lengths of time. The samples were separated by SDS-PAGE and blotted onto PVDF membranes, and p-ERK and total ERK were detected using an anti-phospho-ERK antibody and an anti-ERK antibody, respectively. Representative blots from at least 3 experiments are shown. The relative density of the ERK bands was quantified and is shown in the bar graphs (the mean±SEM) below the individual blots.