Table 1. Potential sites for phosphorylation in CMKLR1.
Position | Kinase | Sequence | Threshold | Risk-Diff | Mutation | Endocytosis |
---|---|---|---|---|---|---|
253 | RLAKTKKPF | T253A | − | |||
314 | NPILYVFMG | Y314A | − | |||
330 | VALFSRLVN | S330A | + | |||
337 | GRK | VNALSEDTG | 2.1 | 3.04 | S337A | + |
343 | GRK | DTGHSSYPS | 2.1 | 3.13 | S343A | − |
344 | PKC | TGHSSYPSH | 2.2 | 2.37 | S344A | + |
347 | PKC | SSYPSHRSF | 2.2 | 3.37 | S347A | − |
350 | PKC | PSHRSFTKM | 2.2 | 3.15 | S350A | + |
352 | PKB | HRSFTKMSS | 3.2 | 4.86 | T352A | + |
The PPSP program34 was used for an analysis of the protein sequence of human CMKLR1. Potential sites for phosphorylation by GRK, PKB (Akt), and PKC were underlined. Selected sites located in the intracellular domains, particularly in the cytoplasmic tail, are listed in the table. The last two columns show the mutations introduced and their effects on receptor internalization.