Table 1. Potential sites for phosphorylation in CMKLR1.

Position	Kinase	Sequence	Threshold	Risk-Diff	Mutation	Endocytosis
253		RLAKTKKPF			T253A	−
314		NPILYVFMG			Y314A	−
330		VALFSRLVN			S330A	+
337	GRK	VNALSEDTG	2.1	3.04	S337A	+
343	GRK	DTGHSSYPS	2.1	3.13	S343A	−
344	PKC	TGHSSYPSH	2.2	2.37	S344A	+
347	PKC	SSYPSHRSF	2.2	3.37	S347A	−
350	PKC	PSHRSFTKM	2.2	3.15	S350A	+
352	PKB	HRSFTKMSS	3.2	4.86	T352A	+

The PPSP program³⁴ was used for an analysis of the protein sequence of human CMKLR1. Potential sites for phosphorylation by GRK, PKB (Akt), and PKC were underlined. Selected sites located in the intracellular domains, particularly in the cytoplasmic tail, are listed in the table. The last two columns show the mutations introduced and their effects on receptor internalization.