Table 2. Actin-Activated MgATPase Rates of NMII Myosins.
Vmax (s–1), KATPase (μM) (from steady-state
ATPasea) |
|||
---|---|---|---|
NMIIA HMM | NMIIB HMM | NMIIC HMM | |
actin | 0.49 ± 0.03, 7.0 ± 1.2 | 0.17 ± 0.02, 3.9 ± 1.2 | 0.23 ± 0.01, 3.9 ± 0.6 |
Tm5NM1 | 0.57 ± 0.03, 1.3 ± 0.3 | 0.19 ± 0.01, 6.2 ± 1.1 | 0.33 ± 0.01, 1.3 ± 0.2 |
The values of Vmax and KATPase from steady-state ATPase assays ± the standard error from three or four experiments for Tm5NM1 and one experiment for actin (Figure 4).