Table 3. Actin Affinities and Thermal Stabilities of Tropomyosins.
| Kappa (×106 M–1) | αHa | TMb (°C) | |
|---|---|---|---|
| AS-Tmstα | 3.5 ± 0.4 | 2.3 ± 0.5 | 44.0 |
| Tm2 | 1.2 ± 0.0 | 5.8 ± 0.7 | 40.0 |
| Tm5a | 1.7 ± 0.1 | 1.8 ± 0.1 | 36.0 |
| Tm5NM1 | 1.8 ± 0.1 | 2.7 ± 0.5 | 42.5 |
| Tm4 | 0.2 ± 0.0 | 2.5 ± 0.5 | 45.5 |
a
The values for the binding constant of Tm to actin, Kapp ± the standard error, and the Hill coefficient, αH ± the standard error, from two or three experiments (Figure 5). The data were fit to the Hill equation, and the Kapp and αH are those reported by Kaleidagraph.
b
TM is the temperature at which the ellipticity at 222 nm, normalized to a scale of 0–1, is equal to 0.5 (Figure 5).