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Transmission electron microscopy of NP1-MNP treated MC3T3-E1 cells. (A) Metal core nanoparticles NP1-MNP (60 μg/mL) were added to MC3T3-E1 cells for 20 h, and TEM was performed. NP1-MNPs were detected in vesicles and free in the cytosol. (B,C) Higher magnification identified NP1-MNP in autolysosomes (merged autophagosome–-lysosome). (D) Pure NP1-MNP by TEM for comparison.
