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Inhibiting autophagosome formation blocks interaction of NP1-MNP with LC3. (A) Schematic of autophagosome and autolysosome formation and sites of inhibitor (3-Methyladenine (3MA), Bafilomycin A1 (BA1), and Chloroquine (CQ)) action. (B) MC3T3-E1 cells were pretreated with the 3MA (0.5 mM), BA1 (0.1 μM), and CQ (10 μM) for 1 h prior to addition of NP1-MNP-PEG for 72 h. The magnetic pulldown assay was used to analyze bound proteins by Western blotting as indicated.
