Table 1. Kinetic Parameters of Tyrosinase in the Presence of 1, Resveratrol, and Kojic Acida.
| compound | Km (M) | Vmax (ΔA490/s) | Ki (M) | |
|---|---|---|---|---|
| none | 2.4 × 10–3 | 4.7 × 10–4 | ||
| 1 | 60 μM | 3.1 × 10–4 | 3.1 × 10–3 | |
| 1 | 120 μM | 2.4 × 10–4 | 2.8 × 10–3 | |
| 1 | 240 μM | 2.0 × 10–4 | 2.5 × 10–3 | |
| resveratrol | 120 μM | 2.5 × 10–4 | 2.9 × 10–3 | |
| kojic acid | 120 μM | 4.3 × 10–4 | 5.5 × 10–3 | |
a
The kinetic parameters are obtained with l-DOPA as a substrate using the Lineweaver–Burk plot. Km (Michaelis constant) and Ki (inhibition constant) values are represented as molar concentration, and Vmax (maximum velocity) values are expressed as the change of absorbance at 490 nm per second.