Figure 27.

Typical single line scan height data of SERCA obtained with different solution conditions. (a) Representative single line scan data obtained at increasing ATP concentration in the range 0–100 μM and in the presence of 10 nM and 100 μM free Ca²⁺. (b) Single line scan data for ∼2 s in the presence of both 1 mM ATP and 100 μm free Ca²⁺. The lower and higher broken lines indicate heights of 5.5 and 8.0 nm measured from the membrane surface, respectively (a, b). Reprinted with permission from ref (49). Copyright 2011 John Wiley & Sons, Inc.