Synergistic hydrolysis
by TrCel6A and TrCel7A visualized by HS-AFM.
(a) Synergy, measured biochemically, between TrCel6A and TrCel7A in
cellobiose production from cellulose IIII. The green and
red plots show the rates of cellobiose production by TrCel6A alone
and TrCel7A alone, respectively, and the blue plot shows the synergy
between the two enzymes. The dotted line indicates the simple sum
of cellobiose production calculated from the green and red plots.
(b) Clips of successive AFM images of cellulose IIII after
injection of TrCel6A at 0 s. The degradation of cellulose was not
discernible even at 8 min. (c) Clips of successive AFM images after
the additional addition of TrCel7A at 0 s (at 8.4 min after the initial
addition of TrCel6A). The cellulose was rapidly degraded and almost
disappeared at 8 min. Imaging rate, 3.3 fps for (b) and (c).