Fig. 1.

HCD increases HNRNPD mRNA and protein levels in animal liver. A, B: Mice fed a HCD (n = 10) or a ND (n = 7) for 4 weeks were euthanized after 4 h fasting. Hepatic gene expression was measured by real-time PCR (A, B). ***P < 0.001 compared with the ND group. C: Individual mouse liver protein extracts were prepared and four lysate samples per group were randomly chosen for Western blotting. D: Expression levels of HNRNPD isoforms, HuR and LDLR, were quantified with the Alpha View software with normalization by signals of β-actin. Values are mean ± SEM of four samples per group. *P < 0.05, **P < 0.01 compared with the ND group. E, F: Male golden Syrian hamsters were fed a HCD (n = 6) or a ND (n = 6) for 2 weeks before euthanization. Hepatic mRNA and protein expressions were determined as described in (A–D).