Fig. 3.
HNRNPD depletion had modest effects on serum cholesterol levels and hepatic LDLR expression levels in mice fed a ND. A–C: Ten C57BL/6J male mice were fed a ND for 2 weeks. Five mice were injected with 6 × 109 pfu/mouse Ad-shHNRNPD; another five mice were injected with 6 × 109 pfu/mouse Ad-shGFP. Four hour-fasted serum samples were collected after 7 days of infection. Serum samples were analyzed for TC (A), LDL-C (B), and HDL-C (C). *P < 0.05 compared with the Ad-shGFP group at the same time points. D: Hepatic mRNA levels of HNRNPD p37/p45 and LDLR were assessed by qRT-PCR using specific primers. After normalization with GAPDH mRNA levels, the relative levels are presented, and the results are the mean ± SEM of four to five animals per group with duplicate measurement of each cDNA sample. ***P < 0.001 compared with the Ad-shGFP group. E: Western blotting with antibodies to HNRNPD p37, HNRNPD p45, and LDLR was conducted as described in Fig. 2E. Values are the mean ± SEM of five samples per group. *P < 0.05, **P < 0.01 compared with the Ad-shGFP group.