Fig. 5.

Induction of HNRNPD mRNA expression by cholesterol in cultured hepatic cell lines. HepG2 cells (A) or AML12 cells (B) were seeded in 6-well culture plates and cultured in medium supplemented with 10% LPDS overnight. The next day, DMSO (as vehicle), cholesterol (10 μg/ml cholesterol + 1 μg/ml 25-hydroxycholesterol), or 5 μM RSV was added to the cells for 24 h prior to isolation of total RNA. Hepatic mRNA levels of HNRNPD, LDLR, and GAPDH were assessed by qRT-PCR using specific primers with triplicate measurement of each cDNA sample. After normalization with GAPDH mRNA levels, the relative levels are presented. The data shown are the summarized results of two separate experiments in which duplicate wells were used in each condition. **P < 0.01, ***P < 0.001 compared with control. CHO, cholesterol. C: HepG2 cells cultured overnight in 10% LPDS medium were treated with RSV, 24-OHC, 24,25-EC, or CHO at the indicated concentrations for 24 h. Gene expression analysis was conducted as in (A) and (B). Duplicate wells were used for each treatment condition.