TABLE 1.
Identification of 35 proteins that specifically purify with the LD
| ORF | Gene | Function | Proteomes Present | Microscopically Localized to LD | Biochemically Localized to LD |
| YIL124W | AYR1 | Acyl-DHAP reductase, catalyzes lyso-PA formation | A, B, G | (69) | (60) |
| YDR196C | CAB5 | Dephospho-CoA kinase involved in CoA synthesis | — | Current Work | — |
| YOR245C | DGA1 | Diacylglycerol acyltransferase, catalyzes DAG to TAG | G | (69) | (63) |
| YHL030W | ECM29 | Proteasome assembly | — | — | — |
| YBR177C | EHT1 | Acyl-CoA:ethanol acyltransferase | A, B, G | (69) | — |
| YGR175C | ERG1 | Squalene epoxidase, enzyme in ergosterol synthesis | A, B, G | (68) | (68) |
| YLR100W | ERG27 | 3-keto sterol reductase, enzyme in ergosterol synthesis | B,G | (69) | (66) |
| YML008C | ERG6 | 24-C-sterol methyltransferase, enzyme in ergosterol synthesis | A, B, G | (68) | (64) |
| YHR072W | ERG7 | Lanosterol synthase, enzyme in ergosterol synthesis | A, B, G | (65) | (65) |
| YOR317W | FAA1 | Fatty acyl-CoA synthetase, activates imported FAs | A, B, G | Current Work | — |
| YMR246W | FAA4 | Fatty acyl-CoA synthetase, activates imported FAs | A, B, G | (69) | — |
| YBR041W | FAT1 | Fatty acyl-CoA synthetase, activates imported FAs | A, B, G | (69) | — |
| YIR038C | GTT1 | Glutathione transferase | B, G | — | (22) |
| YMR110C | HFD1 | Fatty aldehyde dehydrogenase | B, G | Current work | — |
| YBR204C | LDH1 | Serine hydrolase, weak TG lipase activity | G | (70) | (70) |
| YML059C | NTE1 | Phospholipase B | — | — | — |
| YDL193W | NUS1 | Putative prenyltransferase involved in dolichol synthesis | A, G | (43) | — |
| YKR046C | PET10 | Unknown | A, B, G | (69) | — |
| YPL206C | PGC1 | Phosphatidyl glycerol phospholipase C, catalyzes PG to DAG | G | (71) | (72) |
| YGR233C | PHO81 | Cyclin-dependent kinase inhibitor | — | — | — |
| YBR002C | RER2 | Prenyltransferase involved in dolichol synthesis | — | Current work | Current work |
| YGR263C | SAY1 | Sterol deacetylase | — | Current work | Current work |
| YDL052C | SLC1 | Acyltransferase, catalyzes lyso-PA to PA | A, B, G | (69) | (61) |
| YPR140W | TAZ1 | Lyso-PC acyltranferase, catalyzes lyso-PC to PC | — | — | — |
| YKL140W | TGL1 | Lipase, catalyzes SE to sterol | A, B, G | (50) | (73) |
| YMR313C | TGL3 | Lipase, catalyzes TAG to DAG, DAG to MAG, and LPA to PA | A, B, G | (74) | (74) |
| YKR089C | TGL4 | Lipase, catalyzes TAG to DAG, LPA to PA, and SE to sterol | G | (75) | — |
| YOR081C | TGL5 | Lipase, catalyzes TAG to DAG and LPA to PA | G | (75) | (75) |
| YBR265W | TSC10 | 3-Ketosphinganine reductase, involved in sphingosine synthesis | G | Current work | — |
| YML013W | UBX2 | Involved in ER-associated protein degradation, regulates LD homeostasis. | G | (76) | (22) |
| YMR152W | YIM1 | Unknown | A, G | (69) | — |
| YKL094W | YJU3 | Monoacylglyceride lipase, catalyzes MAG to glycerol | A, G | (69) | (67) |
| YKL047W | — | Unknown, putative lipase | — | Current work | — |
| YOR059C | — | Unknown, putative lipase. | A | (69) | — |
| YPR147C | — | Unknown | — | Current work | — |
Identified proteins were found as described in the Results and are annotated for ORF, gene name, presence in other proteomes, and previous localization to the LD by microscopy or biochemistry. Proteomes are abbreviated as A = Athendstaedt et al., 1999 (37), B = Binns et al., 2006 (11), and G = Grillitsch et al., 2011 (22). Proteins were considered previously identified if they were previously microscopically or biochemically localized to the LD or if they were previously identified in more than one proteome. Newly identified proteins are bolded in the table. Proteins that have not been verified by microscopy to localize to the LD in our study or others are in italics. DAG, diacylglycerol; DHAP, dihydroxyacetone phosphate; LPA, lysophosphatidic acid; MAG, monoacyl glycerol; PA, phosphatidic acid; PC, phosphatidyl choline; PG, phosphatidyl glycerol; TAG, triacylglycerol.