TABLE 3.

Association of CpG methylation sites with fasting lipoprotein parameters in the discovery and replication samples and for the whole GOLDN sample

	Markera	Position	Discovery Subsample (N = 663)		Replication Subsample (N = 331)		Full Sample (N = 994)
			β (SE)	P	β (SE)	P	β (SE)b	P
LDL parameters
Concentration of small particles	cg00574958	68607622	−1.3 × 10−5 (1.8 × 10−6)	7.1 × 10−13	−1.1 × 10−5 (2.6 × 10−6)	1.4 × 10−5	−1.92 × 10−4 (2.29 × 10−5)	1.37 × 10−16
Total particles	cg00574958	68607622	−1.3 × 10−5 (2.2 × 10−6)	2.3 × 10−9	−1.2 (7.4 × 10−7)	0.0001	−1.21 × 10−5 (1.72 × 10−6)	3.34 × 10−12
Average diameter	cg00574958	68607622	0.01 (0.001)	4.9 × 10−13	0.01 (0.002)	6.5 × 10−6	0.0078 (9.04 × 10−4)	2.8 × 10−17
VLDL parameters
Concentration of large particles	cg00574958	68607622	−0.001 (1.9 × 10−4)	1.5 × 10−12	−0.001 (2.0 × 10−4)	1.6 × 10−5	−1.42 × 10−4 (1.61 × 10−5)	5.19 × 10−18
	cg17058475	68607737	−0.001 (2.6 × 10−4)	1.8 × 10−7	−0.001 (2.6 × 10−4)	0.0001	−1.57 × 10−4 (2.2 × 10−5)	1.82 × 10−12
Concentration of medium particles	cg00574958	68607622	−2.1 × 10−4 (2.7 × 10−5)	8.0 × 10−15	−2.3 × 10−4 (5.1 × 10−5)	0.0002	−1.24 × 10−4 (1.37 × 10−5)	6.74 × 10−19
	cg17058475	68607737	−3.7 × 10−4 (3.7 × 10−5)	2.6 × 10−11	−1.5 (3.9 × 10−5)	1.4 × 10−5	1.55 × 10−4 (1.86 × 10−5)	2.8 × 10−16
Total particles	cg00574958	68607622	−1.6 × 10−4 (2.0 × 10−5)	1.11 × 10−14	−1.6 × 10−4 (3.7 × 10−5)	6.7 × 10−6	−1.42 × 10−4 (1.52 × 10−5)	6.27 × 10−20
	cg17058475	68607737	−1.7 × 10−4 (2.8 × 10−5)	1.293 × 10−9	−1.1 × 10−4 (2.8 × 10−5)	4.2 × 10−5	−1.73 × 10−4 (2.08 × 10−5)	3.22 × 10−16

For sites that reached genome-wide levels of significance (P < 1.1 × 10⁻⁷) in the discovery cohort and replicated at a Bonferroni corrected α of 0.05 (P < 0.004) in the replication cohort.

^aAll loci are in CPT1A, which is located in Chromosome 11.

^bMean (±SD) for methylation at locus cg00574958 = 0.10 (0.03) and at locus cg17058475 = 0.14 (0.05).