TABLE 2.
Functional characterization of the pike silverside (C. estor) elongase Elovl5 in S. cerevisiae
FA Substrate | FA Product | Individual Conversion (%) | Accumulated Conversion (%) |
18:3n-3 | 20:3n-3 | 35.6 | 41.1 |
22:3n-3 | 5.5 | 5.5 | |
18:2n-6 | 20:2n-6 | 4.9 | 5.2 |
22:2n-6 | 0.3 | 0.3 | |
18:4n-3 | 20:4n-3 | 27.2 | 60.2 |
22:4n-3 | 33.0 | 33.0 | |
18:3n-6 | 20:3n-6 | 27.2 | 27.2 |
22:3n-6 | 0.0 | 0.0 | |
20:5n-3 | 22:5n-3 | 87.1 | 89.6 |
24:5n-3 | 2.5 | 2.5 | |
20:4n-6 | 22:4n-6 | 53.8 | 54.8 |
24:4n-6 | 1.0 | 1.0 | |
22:5n-3 | 24:5n-3 | 1.9 | 1.9 |
22:4n-6 | 24:4n-6 | 0.6 | 0.6 |
Individual conversions were calculated according to the formula [individual product area/(all products areas + substrate area)] × 100. Accumulated conversions were computed by summing the individual conversion for each particular product and also those for longer products.