Skip to main content
. Author manuscript; available in PMC: 2014 Jun 30.
Published in final edited form as: Nat Chem Biol. 2013 Sep 29;9(11):693–700. doi: 10.1038/nchembio.1352

Figure 2. Effects of nicotinic acid (NA), nicotinamide (NAM), and 1-methylnicotinamide (MNA) on C. elegans lifespan in the presence and absence of sir-2.1.

Figure 2

Lifespan analyses of a wild-type (wt) nematodes exposed to 1 µM MNA (red) compared with untreated worms (black); b of wt nematodes exposed to 100 µM NAM (green); c of wt nematodes exposed to 1 mM NA (blue); d of sir-2.1(ok434) nematodes exposed to 1 mM NA; e of sir-2.1(ok434) nematodes exposed to 100 µM NAM; f of sir-2.1(ok434) nematodes exposed to 1 µM MNA; g of sir-2.1 overexpressing nematodes (strain GA468; orange) compared to rol-6 control worms (black); h of sir-2.1 overexpressing nematodes (strain LG389; orange) compared to control worms (strain LG390; black); i of sir-2.1 overexpressing nematodes (strain GA468) exposed to 1 mM NA; j of control LG390 nematodes exposed to 1 mM NA; k of rol-6 control nematodes exposed to 1 mM NA; l of control LG390 nematodes exposed to 1 mM NA. All data were expressed as mean values with n representing the number of independent experiments. Please find further information to statistical analyses in Suppl. Table 1 (also applies to all following C. elegans lifespan assays).