Table 3.
Effect of Unloading and SclAbll Treatment on Static and Dynamic Quantitative Histomorphometry of the Distal Femur
| Controls |
HLU |
|||
|---|---|---|---|---|
| Vehicle | SclAbll | Vehicle | SclAbll | |
| Static indicesa | ||||
| N.Ob/BS (#/mm2) | 24 ± 4 | 23 ± 2 | 26 ± 4 | 32 ± 2 |
| N.Oc/BS (#/mm2) | 3.9 ± 1.1 | 4.4 ± 1.0 | 6.5 ± 1.3 | 5.1 ± 1.3 |
| N.Ot/BA (#/mm2) | 740 ± 44 | 591 ± 34 | 653 ± 93 | 647 ± 38 |
| N.Ad/MA (#/mm2) | 16 ± 4** | 17 ± 4** | 34 ± 7 | 35 ± 6 |
| Ad.Diam (μm) | 44.0 ± 2.5*** | 36.0 ± 1.1** | 51 ± 4 | 50.5 ± 2.4 |
| OS/BS (%) | 24.0 ± 2.0 | 25.7 ± 2.0 | 25.2 ± 5.0 | 33.7 ± 2.0 |
| ES/BS (%) | 6.3 ± 1.5 | 6.3 ± 0.7 | 8.7 ± 1.9 | 10.1 ± 2.4 |
| Dynamic indicesb | ||||
| MS/BS (%) | 13.6 ± 3.3 | 24.9 ± 2.2* | 11.6 ± 5.5 | 21.1 ± 5.0* |
| MAR (|μm/d) | 1.2 ± 0.2** | 1.6 ± 0.2* | 0.5 ± 0.2 | 2.0 ± 1.1* |
| BFR/BS (|μm3/|μm2/d) | 0.2 ± 0.1 | 0.4 ± 0.1* | 0.09 ± 0.1 | 0.9 ± 0.6* |
Values are mean ± SEM. Ad.Diam = adipocyte diameter; BFR/BS = bone formation rate per bone surface; CON = controls; ES/BS = eroded surface per bone surface; HLU = hindlimb unloaded; MAR = mineral apposition rate; MS/BS = mineralizing surface; N.Ad/MA = adipocyte number per marrow area; N.Ob/ BS = osteoblast number per bone surface; N.Oc/BS = osteoclast number per bone surface; N.Ot/BA = osteocyte number per bone area; OS/BS = osteoid osteoid surface per bone surface; SclAbll = sclerostin antibody; VEH = vehicle.
Sample size for static indices: n = 6/group.
Sample size for dynamic indices: CON-VEH, n = 6; CON-SclAbll, n = 5; HLU-VEH, n = 3; HLU-SclAbll, n = 3.
p < 0.05 SclAbll versus VEH treatment within the CON or HLU groups.
p < 0.05 HLU versus CON within the SclAbll and VEH treatment groups.
p = 0.08 HLU versus CON within VEH-treated group.